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. 2020 Apr 3;11:552. doi: 10.3389/fmicb.2020.00552

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Copyright © 2020 Mathew, Torky, Amin, Mourad, Ayyash, El-Keblawy, Hilal-Alnaqbi, AbuQamar and El-Tarabily.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of Streptomyces tritolerans RAK1 based on phylogenetic, cultural and morphological characteristics. (A) The tree showing the phylogenetic relationships between the polyamine-producing S. tritolerans RAK1 (isolate #7; MN795132; 1,516 bp) and other members of Streptomyces spp. on the basis of 16S rRNA sequences. (B) Aerial mycelia (left) and substrate mycelia (right) growing on ISP3 medium supplemented with yeast extract; and (C) scanning electron micrograph (6,000X) showing closed straight to flexuous (rectus-flexibilis) chains of spores (red arrow) with smooth spore structure (yellow arrow) of the strain of S. tritolerans RAK1. In (A) numbers at nodes indicate percentage levels of bootstrap support based on a neighbor-joining analysis of 1000 resampled datasets. Bar, 0.001 substitutions per site. GenBank accession numbers are given in parentheses.