Table 2.
Verification of differential gene expression in offspring from microarray (Mouse Clariom™ D assay) on pooled samples by RT-PCR at individual level.
| Mouse Clariom™ D assay | RT-PCR (n-control, 14; n-bbp, 10) | |||||
|---|---|---|---|---|---|---|
| Probe Set ID | Gene | RNA-Pool | Ensembl-ID | BBP vs. Control | ||
| x-fold | ENSMUSG000000 | p-value (ttest) | x-fold | Reference for “Arthritis” | ||
| TC0100003029.mm.1 | cxcr4 | 2.1 | 45382 | 0.002 | 1.7 | (21) |
| TC1000002224.mm.1 | ddit4 | 2.4 | 20108 | 0.009 | 2.0 | (22)* |
| TC1800000301.mm.1 | egr1 | 2.5 | 38418 | 0.25 | 1.7 | (23) |
| TC1500001197.mm.1 | il7r | 2.5 | 3882 | 7.8E-05 | 1.8 | (24, 25) |
| TC0100003240.mm.1 | prg4 | 3.9 | 6014 | 0.020 | 3.5 | (26) |
| TC0100003207.mm.1 | rgs1 | 2.9 | 24042 | 6.3E-06 | 3.2 | (27) |
| TC0100001381.mm.1 | rgs16 | 2.1 | 26475 | 0.23 | 1.4 | |
| TC1700001827.mm.1 | sik1 | 2.6 | 24042 | 3.4E-06 | 2.1 | (28) |
| TC1000001813.mm.1 | tnfaip3 | 3.1 | 19850 | 0.09 | 1.5 | (29, 30) |
| TC1100003093.mm.1 | alox15 | −2.9 | 18924 | 0.11 | 1.5 | (31)* |
| TC0300001667.mm.1 | car1 | −2.5 | 27556 | 0.94 | −1.0 | |
| TC1100003399.mm.1 | ccl6 | −2.3 | 18927 | 0.84 | −1.0 | |
| TC0500003382.mm.1 | cldn13 | −3.4 | 8843 | 0.12 | −1.9 | |
| TC1600000763.mm.1 | cpox | −2.2 | 22742 | 0.25 | −1.3 | |
| TC0500002755.mm.1 | cxcl9 | −2.3 | 29417 | 0.19 | −1.2 | |
| TC0800000971.mm.1 | klf1 | −2.8 | 54191 | 0.055 | −2.0 | |
| TC1400000322.mm.1 | nt5dc2 | −2.6 | 71547 | 0.007 | −1.4 | |
| TC0300000747.mm.1 | pklr | −3.2 | 41237 | 5.4E-05 | −2.4 | |
Gene expression was measured on fourteen 3-week-old mice from control and ten mice from BBP-exposed dams. RT-PCR overall confirms direction of gene expression and partially reaches significant differences between groups. Remarkably, genes associated with arthritis were enriched.
*
Association of downregulation with arthritis.
Statistical significance is indicated in bold (p < 0.05).