Figure 2.

CRISPR/Cas9 genome editing genetically depleted Foxp1in A20 cells. (A) Exons 5, 6, and 7 of Foxp1 were selected for CRISPR/Cas9 targeting. gRNA sequences are shown in bold and underlined. (B) Individual CRISPR clones were grown from sorted A20 cells after CRISPR/Cas9 targeting. Most clones showed loss of the full length Foxp1 protein (Foxp1_L) by Western blotting. Exon 5 targeting did not deplete the Foxp1 short (Foxp1_S proteins). Exon 6 targeting reduced expression of the higher molecular weight Foxp1 Foxp1_S isoform and several Exon 7 targeted clones no longer expressed any Foxp1. Nucleophosmin (Npm) or β-actin was used as a sample loading control.