Figure 6.

Individual genes most affected by Foxp1 knockout include the B-cell marker Cd19. (A) Quantitative PCR independently validates reduced gene expression of Cd19, Iglv2, and Phyhip and Lgr6 in Foxp1 depleted A20 clones. **p < 0.005, ***p < 0.001, ****p < 0.0001. Gene expression for each clone was compared with that of the A20 parental cell line using Student's t-test. (B) Flow cytometry analysis confirms that Cd19 protein expression at the cell surface expression is reduced in Foxp1 knockout cells. (C) Gene set enrichment analysis shows that genes in specific hallmark signatures involving Myc and oxidative phosphorylation are affected by Foxp1 depletion. The red vertical line on the lower right panel indicates FDR adjusted p-value p < 0.05. The estrogen response early pathway is labeled as having a neutral direction of regulation because it is upregulated in the E7 A20 clones and downregulated in the E6 A20 clones.