Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Several approaches have been used to improve the function of Cas9 proteins and reduce off-target effects. Cas9 function has been modified through the rational design and engineering of a higher fidelity nuclease, modifying the sgRNA for increased stability, directed evolution toward hyperaccuracy, or fusing Cas9 with programmable DNA-binding domains.
