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. 2020 Apr 3;8:230. doi: 10.3389/fcell.2020.00230

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Copyright © 2020 Ji, Pan, Shen, Yang, Wang, Bai and Tao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing CircRNA124534 suppresses the osteogenic differentiation of DPSCs and overexpressing β-catenin reversed the inhibition. (A) The level of CircRNA124534 and β-catenin was determined by RT-PCR in DPSCs transfected with CircRNA124534 silencing vector (si-circ) or β-catenin overexpression vector (oe-β-catenin) for 48 h. (B) The level ofβ-catenin was determined by western blotting in DPSCs transfected with si-circ or oe-β-catenin for 48 h. (C,D) ARS mineralization assay on day 14 after osteogenic induction in PM or OM. Scale bars = 50 μm. (E,F) ALP activity on day 7 after osteogenic induction. Scale bars = 100 μm. (G,H) The levels of RUNX and OCN were determined by RT-PCR and western blotting in DPSCs on day 14 after osteogenic induction. Data indicate the mean ± SD, n = 3. **P < 0.01, ***P < 0.001 vs. control, ^##P < 0.01, ^###P < 0.001 vs. miR-496 inhibitor.