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. 2020 Apr 3;11:386. doi: 10.3389/fphar.2020.00386

Figure 3.

Figure 3

Trichomicin inhibited phosphorylation of components of the NF-κB signaling pathway. (A) Effect of Trichomicin on TNFα–induced phosphorylation of NF-κB p65, IKKα/β, IκBα and basal Stat3 phosphorylation in HCT-116 cells. (B) The effect of Trichomicin on the NF-κB and Stat3 signaling pathway in HCT-116 transfected with NF-κB p65 siRNA. (C) Immunoblotting analysis of TNFα–induced phosphorylation of NF-κB p65 and basal Stat3 phosphorylation in HCT-116 cells treated with BP-1-102. HCT-116 cells were preincubated with Trichomicin or BP-1-102 for 1 h prior to treatment with TNFα for 1 h. Whole cell lysates were prepared for western blotting. (n≥3) *P < 0.05, **P < 0.01, ***P < 0.001.