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. 2020 Feb 26;12(3):610. doi: 10.3390/nu12030610

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of fractions of 5-uRCK or ellagic acid on cholesterol accumulation in FFA-induced HepG2 cells. HepG2 cells were treated with a 1 mM FFA mixture (oleic acid/palmitic acid, 2:1) and various concentrations of 5-uRCK fractions or ellagic acid for 24 h. (A) The effect of various fractions of 5-uRCK or 5-uRCC on the intracellular total cholesterol content in FFA-induced HepG2 cells. (B) The effect of the ethyl acetate fraction of 5-uRCK on the intracellular total cholesterol content in FFA-induced HepG2 cells. (C) The effect of purified ellagic acid from 5-uRCK on the intracellular total cholesterol content in FFA-induced HepG2 cells. ** p < 0.01, *** p < 0.001 versus control (CTL). ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 versus FFA-treated cells. The data are represented as the means ± SEMs.