Table 3.
Species significantly influential for being classified into cluster H1, H2 or H4, respectively, when compared with cluster H3. Species in bold are known to be acidogenic and acid-tolerant species. Data for cluster H3 (lowest sucrose intake) against the three other clusters simultaneously are found in Table S4.
| Cluster H1 (n = 70) | Cluster H2 (n = 33) | Cluster H4 (n = 24) |
|---|---|---|
| model R2 = 50%, Q2 = 50% | model R2 = 84%, Q2 = 63% | model R2 = 80, Q2 = 53 |
| sucrose intake = 6.5 E% | sucrose intake 6.4 E% | sucrose intake 6.0 E% |
| DeFS = 5.8 | DeFS = 4.5 | DeFS = 5.1 |
| sucrose, E% | sucrose, E% | sucrose, E% |
| sugar, E% | sugar, E% | sugar, Eproc |
| milk 3% | milk, 1,5% | |
| monosaccharides, E% | ||
| Actinomyces sp. HMT171 | Actinomyces israelii | Actinomyces odontolyticus |
| Actinomyces sp. HMT178 | Actinomyces massiliensis | Actinomyces sp. HMT171 |
| Alloprevotella sp. HMT308 | Actinomyces sp. HMT171 | Actinomyces sp. HMT448 |
| Alloscardovia omnicolens | Actinomyces sp. HMT178 | Aggregatibacter sp. HMT458 |
| Bifidobacterium longum | Actinomyces sp. HMT897 | Alloprevotella sp. HMT308 |
| Capnocytophaga sp. HMT326 | Aggregatibacter sp. HMT458 | Bacteroidales [G-2] bacterium HMT274 |
| Capnocytophaga sp. HMT902 | Aggregatibacter sp. HMT949 | Bacteroidetes [G-5] bacterium HMT505 |
| Dietzia cinnamea | Alloprevotella sp. HMT912 | Bacteroidetes [G-5] bacterium HMT511 |
| Lachnoanaerobaculum orale | Alloprevotella sp. HMT913 | Capnocytophaga sp. HMT326 |
| lactobacilli, culture | Atopobium rimae | Capnocytophaga sp. HMT338 |
| Leptotrichia wadei | Bacteroidales [G-2] bacterium HMT274 | Corynebacterium singulare |
| Megasphaera micronuciformis | Bacteroidetes [G-3] bacterium HMT281 | Dialister invisus |
| mutans streptococci, culture | Bacteroidetes [G-5] bacterium HMT511 | Dialister pneumosintes |
| Olsenella sp. HMT807 | Bergeyella sp. HMT206 | Fusobacterium nucleatum ssp. animalis |
| Peptostreptococcaceae [XI][G-7] yurii | Bergeyella sp. HMT907 | Granulicatella elegans |
| Prevotella histicola | Bifidobacterium dentium | Haemophilus parahaemolyticus |
| Prevotella sp. HMT305 | Butyrivibrio sp. HMT080 | Kingella oralis |
| Prevotella sp. HMT306 | Campylobacter gracilis | Lachnoanaerobaculum orale |
| Prevotella sp. HMT313 | Capnocytophaga granulosa | Lactobacillus crispatus |
| Prevotella sp. HMT317 | Capnocytophaga haemolytica | Leptotrichia sp. HMT221 |
| Scardovia wiggsiae | Capnocytophaga ochracea | Leptotrichia wadei |
| Stomatobaculum longum | Capnocytophaga sp. HMT326 | Megasphaera micronuciformis |
| Streptococcus intermedius | Capnocytophaga sp. HMT332 | Mycoplasma faucium |
| Streptococcus mutans | Capnocytophaga sp. HMT338 | Neisseria bacilliformis |
| Streptococcus parasanguinis clade411 | Capnocytophaga sp. HMT903 | Olsenella sp. HMT807 |
| Veillonella atypica | Cardiobacterium valvarum | Peptococcus sp. HMT167 |
| Veillonella dispar | Catonella sp. HMT164 | Peptostreptococcaceae [XI][G-5] saphenum |
| Dialister pneumosintes | Peptostreptococcaceae [XI][G-9] brachy | |
| Eikenella corrodens | Porphyromonas endodontalis | |
| Fusobacterium hwasookii | Prevotella denticola | |
| Fusobacterium naviforme | Prevotella histicola | |
| Fusobacterium nucleatum subsp. animalis | Prevotella intermedia | |
| Fusobacterium nucleatum subsp. polymorphum | Prevotella sp. HMT305 | |
| Fusobacterium sp. HMT204 | Prevotella sp. HMT306 | |
| Gemella morbillorum | Prevotella sp. HMT317 | |
| Haemophilus haemolyticus | Saccharibacteria (TM7) [G-5] bacterium HMT356 | |
| Johnsonella sp. HMT166 | Scardovia wiggsiae | |
| Kingella denitrificans | Streptococcus mutans | |
| Kingella oralis | Streptococcus parasanguinis clade 411 | |
| Kingella sp. HMT012 | Streptococcus sobrinus | |
| Lachnoanaerobaculum saburreum | Streptococcus sp. HMT057 | |
| Leptotrichia buccalis | Tannerella forsythia | |
| Leptotrichia shahii | Treponema denticola | |
| Leptotrichia sp. HMT219 | Treponema lecithinolyticum | |
| Leptotrichia sp. HMT223 | Treponema socranskii | |
| Leptotrichia sp. HMT392 | Treponema sp. HMT237 | |
| Leptotrichia sp. HMT498 | Veillonella atypica | |
| Leptotrichia wadei | Veillonella dispar | |
| Mycoplasma salivarium | GNAT3 (rs11760281 | |
| Olsenella sp. HMT807 | ||
| Oribacterium sp. HMT078 | ||
| Ottowia sp. HMT894 | ||
| Parvimonas micra | ||
| Peptococcus sp. HMT167 | ||
| Peptostreptococcaceae [XI][G-5] saphenum | ||
| Peptostreptococcaceae [XI][G-7] bacterium HMT081 | ||
| Peptostreptococcaceae [XI][G-7] yurii | ||
| Porphyromonas catoniae | ||
| Porphyromonas sp. HMT275 | ||
| Porphyromonas sp. HMT278 | ||
| Prevotella fusca | ||
| Prevotella intermedia | ||
| Prevotella maculosa | ||
| Prevotella micans | ||
| Prevotella nigrescens | ||
| Prevotella oulorum | ||
| Prevotella pleuritidis | ||
| Prevotella saccharolytica | ||
| Prevotella sp. HMT300 | ||
| Prevotella sp. HMT301 | ||
| Prevotella sp. HMT317 | ||
| Prevotella sp. HMT472 | ||
| Prevotella sp. HMT475 | ||
| Rothia aeria | ||
| Saccharibacteria (TM7) [G-1]bacterium HMT348 | ||
| Saccharibacteria (TM7) [G-5] bacterium HMT356 | ||
| Selenomonas noxia | ||
| Stomatobaculum longum | ||
| Streptococcus constellatus | ||
| Streptococcus gordonii | ||
| Streptococcus intermedius | ||
| Streptococcus parasanguinis clade 411 | ||
| Tannerella forsythia | ||
| Treponema socranskii | ||
| Treponema sp. HMT237 | ||
| Treponema sp. HMT246 | ||
| Treponema sp. HMT262 |
Taxa in Cluster H1 (high sucrose intake) compared with H3 (lowest sugar intake) from LEfSe analysis is further illustrated in a clade diagram (Figure 5A) with effect sizes in an LDA histogram (Figure 5B). Species in the phyla Streptococcus and Actinomyces were associated with H1. At the species level, largely the same species as identified by PLS appeared. The strongest effect sizes (LDA scores of about 4) were seen for species in the genera Streptococcus and Prevotella (Figure 5B).