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. 2020 Mar 2;12(3):677. doi: 10.3390/nu12030677

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of autophagy inhibitor 3-MA and 3DC2ME isolated from Z. jujuba root extract on expression of (A) LC3, Bax, Bcl-2 and cleaved caspase-3 in LLC-PK1 cells treated with 25 μM cisplatin by western blotting in the presence or absence of 3-MA. (B–E) Bar graphs indicated the relative ratio of the western blot band signals in each group as compared with control cells (mean ± SD, * p < 0.05 cisplatin-treated LLC-PK1 cells). 3DC2ME, 3-dehydroxyceanothetric acid 2-methyl ester; 3-MA, 3-methyladenine; SD, standard deviation.