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Glycine supplementation restored mucin in LPS-treated mice. (A) Alcian blue staining; (B,C) relative mRNA levels of Muc5ac and Muc5b. Red arrows indicate goblet cells. White and black scale bars mean 50 and 20 μm respectively. The mRNA level in the CON group was set as 1.00 to calibrate the relative levels. Gapdh was used as a reference gene. Values are means ± SEM, n = 7. Means for an indicated parameter without a common letter differ, p < 0.05.
