Figure 5.

Permeability of total antioxidants (a) and specific polyphenols (HTS (b), TS (c), and OLE (d)) from OPEs bioaccessible fractions and mix. Polyphenols’ amount and TEAC (Trolox equivalent antioxidant capacity) were determined in both, apical and basolateral chamber and expressed as percentage of the amount applied on cell monolayer. The difference between the amount found in the apical and basolateral chamber and the initial amount was expressed as metabolized fraction representing the amount that is either metabolized or accumulated inside cells. Initial concentrations were: 14 mg/mL for nat (native OP); 28 mg/mL for bCD (OP + β CD), hpbCD (OP + hydroxypropyl β CD), ramCD (OP + randomly methylated β CD), gCD (OP + γ CD); 20 μg/mL for mix (hydroxytyrosol, tyrosol, oleuropein); data are presented as mean ± standard deviation. All the experiments were done in triplicate; OPE (olive pomace extract), bf (bioaccessible fraction), CD (cyclodextrin).