Figure 6.

Determination of antioxidative effect of OPEs bioaccessible fractions and mix of polyphenols. (a) mass concentration and polyphenol composition of each OPE administered in each well with confluent Caco-2 monolayer. (b) Caco-2 cell viability determined by the MTT assay when treated with HBSS (ctr+); OPE and tBOOH (nat_bf, bCD_bf, hpbCD_bf, ramCD_bf, gCD_bf); HTS, TS, and OLE, and tBOOH (mix); and tBOOH (ctr-). Cell viability was expressed as fold change calculated by Equation (2). Data was expressed as mean ± standard deviation. All the experiments were done in 3 replicates; HTS (hydroxytyrosol), TS (tyrosol), OLE (oleuropein), mix (HTS + TS + OLE), bf (bioaccessible fraction), CD (cyclodextrin), nat (native OP), bCD (OP + β CD), hpbCD (OP + hydroxypropyl β CD), ramCD (OP + randomly methylated β CD), gCD (OP + γ CD), OPE (olive pomace extract), tBOOH (tert-butyl hydroperoxide).