Scattered plot of relative abundance of stool bacteria versus relative increase of body weight. Genera Akkermansia (A), Bacteroides (B), unknown genus in the family Enterobacteriaceae (C), and unknown genus in the family Helicobacteraceae (D), were analyzed by 16S metagenome sequencing and plotted versus relative increase of body weight. Only Akkermansia muciniphila was analyzed by qPCR (E). Open triangle, CONT group; closed circle, BTS group. Increase of body weight (%) was calculated as follows: 100 × ((body weight at Week 4) − (body weight at Week 0))/(body weight at Week 0).