Figure 1.

Feeding of diet deficient in vitamin B₂, B₆, B₁₂, and folic acid induced accumulation of glycolate in plasma, cecum, and luminal content of the cecum. (A) Study design for Experiment 1. Mice were fed a control diet (C: AIN93-G) or a diet deficient in vitamin B₂, B₆, B₁₂, and folic acid (VB-) diet for 2 or 4 weeks. (B,C) Volcano plots of 77 metabolites in plasma from mice fed either VB- or control diet for (B) 2 or (C) 4 weeks. The glycolate (GA) concentration changed significantly in both the 2- and 4-week feeding groups. (D–F) Relative concentration of glycolate in (D) plasma, (E) cecum, and (F) luminal content for the four groups (n = 4–5). Two-way ANOVA was used to determine the effect of diet, time, and the interaction of diet and time; p < 0.05.