Figure 2.

Both the gut microbiota and host accumulated glycolate in mice fed the VB- diet. (A) Study design for Experiment 2. Mice were fed the control diet or VB- diet for 4 weeks with or without treatment with four different antibiotics (150 mg/mouse/day penicillin V, ampicillin, metronidazole, and 75 mg/mouse/day vancomycin solved in distilled water). (B–E) Relative concentration of GA in (B) luminal content, (C) cecum, (D) plasma, and (E) stool among the four groups (n = 3–4). Two-way ANOVA was used to determine the effect of diet, antibiotics, and the interaction of the two. p < 0.05. (F) Spearman’s rank correlation coefficient used to describe correlation between luminal GA concentration and that in cecum, plasma, and stool had values of 0.95, 0.61, and 0.12, respectively.