Figure 3.

Feeding of a vitamin B₆-deficient diet did not induce glycolate accumulation in plasma or luminal content. (A) Study design for Experiment 3. Mice were fed the control diet, VB- diet, or vitamin B₆-deficient (VB6-) diet for 2 weeks with or without VB6 supplementation (6 μg/mL pyridoxine hydrochloride solution in distilled water). (B) Average daily VB6 intake and (C) plasma VB6 concentration for the five groups. (D,E) Relative concentration of GA in (D) plasma and (E) luminal content for the five groups. (F) Spearman’s rank correlation coefficients between plasma VB6 concentration and plasma GA levels (R² = 0.1035, p > 0.1). ANOVA was used to determine the differences in each group (n = 4). Different letters indicate p < 0.05.