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. 2020 Mar 20;12(3):831. doi: 10.3390/nu12030831

Figure 1.

Figure 1

Bone marrow-dendritic cells (BM-DCs) pulsed with ovalbumin with pepsin (OP) acquire tolerogenic features. BM-DCs from naïve mice were cultured for 24 h without stimuli (RPMI), or with OP, retinoic acid (RA), IL-4, OP+IL-4, or RA+IL-4. Gene expression of Aldh1a2, Tgfb1, Il6, Csf2, Il10, Il27, IL33, Jag2, Dll4, Tnfsf4, Irf4, and Irf8 was assayed by quantitative polymerase chain reaction (qPCR), normalised to the reference gene Actb, and expressed relative to BM-DCs cultured in RPMI. Results of an experiment representative of, at least 3, separate experiments performed in triplicate are shown. Data are means ± standard error of the mean (SEM). Different letters indicate statistically significant differences (p < 0.05) calculated using the Mann–Whitney U test.