Table 1.
DNA concentration, Illumina sequencing reads and amplicon sequence variants (ASVs) during colonic fermentation of negative control (NegativeC), positive control (PositiveC), non-UV-C irradiated mango bar (MangoB) and UV-C irradiated mango bar (UVMangoB) treatments 1.
| Treatment/ Time (h) | DNA (ng/µL of sample) 2 | Ratio 260/280 | Paired-End Reads | Number of ASVs | Number of Reads into ASVs |
|---|---|---|---|---|---|
| NegativeC | |||||
| 0 | 3.33 ± 1.26 A, c | 1.36 ± 0.11 | 37,369 ± 44,721 | 186 ± 75 | 17,634 ± 22,852 |
| 6 | 4.12 ± 1.26 A, b | 1.50 ± 0.05 | 36,373 ± 12,886 | 192 ± 82 | 14,331 ± 3447 |
| 12 | 6.51 ± 3.65 A, c | 1.61 ± 0.32 | 46,199 ± 9255 | 256 ± 88 | 22,897 ± 27,685 |
| 24 | 5.69 ± 1.25 A, b | 1.46 ± 0.06 | 23,887 ± 9255 | 257 ± 39 | 9459 ± 3742 |
| 48 | 4.46 ± 1.43 A, b | 1.50 ± 0.01 | 32,985 ± 9351 | 290 ± 26 | 12,738 ± 3886 |
| PositiveC | |||||
| 0 | 8.10 ± 2.69 C, a | 1.79 ± 0.20 | 33,125 ± 18,765 | 239 ± 31 | 12,461 ± 7373 |
| 6 | 33.24 ± 20.33 B,C, a | 1.99 ± 0.22 | 26,111 ± 15,951 | 191 ± 32 | 11,510 ± 8355 |
| 12 | 22.56 ± 8.57 C, b,c | 1.99 ± 0.08 | 28,090 ± 10,072 | 210 ± 27 | 12,720 ± 4653 |
| 24 | 77.84 ± 41.85 B, a,b | 2.09 ± 0.04 | 40,410 ± 5110 | 241 ± 18 | 17,432 ± 3531 |
| 48 | 173.80 ± 37.93 A, a | 2.10 ± 0.07 | 37,155 ± 4676 | 175 ± 24 | 19,665 ± 2817 |
| MangoB | |||||
| 0 | 3.02 ± 0.46 C, c | 1.51 ± 0.42 | 21,467 ± 13,471 | 212 ± 84 | 9405 ± 5675 |
| 6 | 27.21 ± 3.04 C, a | 2.01 ± 0.05 | 25,853 ± 9343 | 184 ± 16 | 12,780 ± 4895 |
| 12 | 70.015 ± 37.54 B,C, a | 2.09 ± 0.11 | 23,745 ± 14,122 | 196 ± 26 | 10,960 ± 6581 |
| 24 | 155.96 ± 65.02 B, a | 2.18 ± 0.05 | 30,332 ± 7471 | 189 ± 23 | 12,939 ± 3135 |
| 48 | 249.02 ± 83.25 A, a | 2.19 ± 0.02 | 22,516 ± 7369 | 217 ± 34 | 8927 ± 3325 |
| UVMangoB | |||||
| 0 | 6.37 ± 3.55 C, b,c | 1.78 ± 0.11 | 13,274 ± 1153 | 129 ± 112 | 3538 ± 3080 |
| 6 | 24.30 ± 5.19 C, a | 7.80 ± 0.11 | 20,042 ± 11,747 | 179 ± 27 | 9578 ± 5704 |
| 12 | 53.27 ± 14.96 C, a,b | 1.84 ± 0.22 | 28,441 ± 5716 | 225 ± 17 | 13,631 ± 2283 |
| 24 | 134.00 ± 64.64 B, a | 2.15 ± 0.07 | 29,500 ± 3691 | 262 ± 8 | 12,447 ± 1313 |
| 48 | 196.82 ± 21.88 A, a | 2.18 ± 0.02 | 25,614 ± 2438 | 285 ± 8 | 10,648 ± 1041 |
1 Mean values ± standard deviation (n = 3). 2 Significant differences (p < 0.05) using ANOVA/Fisher’s least significant difference (LSD) test, are indicated in column by different capital letters (A, B, C) within monitoring times for each treatment. Lowercase letters (a, b, c) indicated in same column significant differences (p < 0.05) between similar monitoring times of treatments.