Table 4.
Fatty acid content (%) in heart fractions obtained with the Bodennec et al. [63] method.
| Neutral lipids | Ceramides | Glycosphingolipids | Sphingomyelins | |||||
|---|---|---|---|---|---|---|---|---|
| SD | HFD | SD | HFD | SD | HFD | SD | HFD | |
| 16:0 | 26.2 ± 2.02 | 24.1 ± 2.08 | 29.6 ± 4.12 | 24.3 ± 0.54 | 43.0 ± 4.14 | 33.7 ± 0.79 * | 24.1 ± 2.13 | 22.3 ± 1.71 |
| 18:0 | 8.51 ± 1.38 | 8.59 ± 0.43 | 16.1 ± 2.74 | 11.9 ± 1.00 * | 34.5 ± 2.80 | 31.2 ± 1.85 | 24.6 ± 1.34 | 25.6 ± 1.29 |
| Other SFAs | 6.38 ± 0.62 | 5.56 ± 0.42 | 12.9 ± 2.43 | 8.61 ± 1.81 | 6.02 ± 2.45 | 4.64 ± 0.25 | 2.11 ± 0.12 | 1.64 ± 0.16 ** |
| SFAs | 41.1 ± 1.26 | 38.3 ± 2.33 | 58.6 ± 8.77 | 44.8 ± 2.91 * | 83.5 ± 4.68 | 69.6 ± 1.43 ** | 50.8 ± 3.13 | 49.6 ± 2.97 |
| 16:1 | 8.28 ± 0.99 | 3.99 ± 0.58 ** | 4.98 ± 0.58 | 4.19 ± 0.69 | 1.55 ± 0.38 | 1.53 ± 0.74 | 1.34 ± 0.09 | 0.54 ± 0.05 # |
| 18:1 | 44.0 ± 1.14 | 42.7 ± 2.02 | 29.8 ± 7.31 | 35.5 ± 2.93 | 9.02 ± 1.70 | 10.6 ± 0.95 | 22.6 ± 1.85 | 14.4 ± 0.52 # |
| Other MUFAs | 2.79 ± 0.63 | 1.59 ± 0.25 | 2.52 ± 0.52 | 1.53 ± 0.23 * | 0.31 ± 0.11 | 0.17 ± 0.01 | 0.47 ± 0.08 | 0.28 ± 0.03 ** |
| MUFAs | 55.1 ± 1.25 | 48.2 ± 2.00 * | 37.3 ± 7.89 | 41.2 ± 2.03 | 10.9 ± 1.32 | 12.3 ± 1.63 | 24.4 ± 1.90 | 15.2 ± 0.57 # |
| 18:2 n-6 (LA) | 3.43 ± 1.26 | 9.70 ± 0.10 ** | 2.71 ± 0.82 | 8.86 ± 1.27 # | 1.74 ± 0.68 | 4.20 ± 0.30 ** | 6.00 ± 0.96 | 8.15 ± 1.55 * |
| 20:4 n-6 (ARA) | 0.30 ± 0.27 | 1.20 ± 0.14 * | 0.75 ± 0.35 | 1.99 ± 0.29 ** | 2.21 ± 1.34 | 6.63 ± 0.21 ** | 9.07 ± 1.66 | 12.4 ± 1.09 * |
| 22:4 n-6 (AdA) | 0.07 ± 0.05 | 0.47 ± 0.06 ** | 0.05 ± 0.03 | 0.37 ± 0.02 # | 0.07 ± 0.04 | 0.36 ± 0.01 # | 0.33 ± 0.07 | 0.70 ± 0.07 # |
| Other n-6 PUFAs | 0.19 ± 0.12 | 0.90 ± 0.17 ** | 0.23 ± 0.09 | 0.59 ± 0.11 ** | 0.50 ± 0.40 | 0.90 ± 0.10 | 2.98 ± 0.71 | 2.34 ± 0.14 |
| 18:3 n-3 (ALA) | 0.02 ± 0.01 | 0.02 ± 0.01 | 0.06 ± 0.02 | 0.04 ± 0.01 | 0.04 ± 0.02 | 0.02 ± 0.01 | 0.01 ± 0.01 | 0.02 ± 0.01 |
| 20:5 n-3 (EPA) | 0.22 ± 0.15 | 0.16 ± 0.04 | 0.4 ± 0.23 | 0.18 ± 0.06 | 0.43 ± 0.17 | 0.09 ± 0.02 * | 1.39 ± 0.24 | 0.18 ± 0.02 # |
| 22:5 n-3 (DPA) | 0.12 ± 0.10 | 1.01 ± 0.14 ** | 0.08 ± 0.04 | 0.88 ± 0.07 # | 0.15 ± 0.12 | 1.35 ± 0.16 # | 0.72 ± 0.20 | 2.57 ± 0.39 |
| 22:6 n-3 (DHA) | 0.12 ± 0.08 | 0.84 ± 0.12 ** | 0.32 ± 0.13 | 1.39 ± 0.07 # | 1.07 ± 0.86 | 4.81 ± 0.23 # | 4.75 ± 1.43 | 9.08 ± 0.64 # |
| Other n-3 PUFAs | 0.013 ± 0.005 | 0.02 ± 0.01 | 0.010 ± 0.007 | 0.03 ± 0.01 * | 0.02 ± 0.01 | 0.04 ± 0.01 | 0.10 ± 0.02 | 0.09 ± 0.03 # |
| PUFA | 4.47 ± 2.01 | 14.3 ± 0.70 ** | 4.61 ± 1.61 | 14.3 ± 1.04 # | 6.24 ± 3.57 | 18.4 ± 0.37 ** | 25.4 ± 5.05 | 35.5 ± 3.43 * |
p-value from t-tests: * p < 0.05, ** p < 0.01, # p < 0.001; AdA–adrenic acid (22:4 n-6); ALA–α-linolenic acid (18:3 n-3); ARA–arachidonic acid (20:4 n-6); DHA–docosahexaenoic acid (22:6 n-3); DPA–docosapentaenoic acid (22:5 n-3); EPA–eicosapentaenoic acid (20:5 n-3); LA–linoleic acid (18:2 n-6); MUFA–monounsaturated fatty acids; PUFA–polyunsaturated fatty acids; SFA–saturated fatty acids. Bold represents main groups of fatty acids. SD – mice fed standard diet; HFD – mice fed high fat diet