Skip to main content
. 2020 Mar 1;12(3):673. doi: 10.3390/nu12030673

Figure 8.

Figure 8

Citrus peel inhibits ER stress in AML12 cells. AML12 cells were treated with the indicated concentrations of 25, 50, and 100 μg/mL citrus peel, metformin for 24 h for indicated periods, and (A) immunoblotting was performed using antibodies against p-mTOR, mTOR, p-p70S6K, p70S6K, GRP78, CHOP, p-PERK, PERK, p-eIF2α, eIF2α, and β-actin. (B) Immunoblotting was performed using antibodies against SREBP-1, FAS, p-AMPK, AMPK and β-actin. (C) mRNA expression levels including stearoyl-CoA desaturase-1 (SCD-1), 3-hydroxy-3-methylglutaryl CoA reductase (HMG-R), glycerol 3-phosphate acyltransferase (GPAT), and acetyl-coenzyme A carboxylase (ACC) were measured as described in Materials and Methods. CPEW, citrus peel extract hot-air dry; CPEF, citrus peel extract freeze-drying.