Figure 2.
Effect of WPE on cytotoxicity of MT or CPF in splenic lymphocytes subpopulations. Splenocytes were treated with (A) MT (10−5 M) or CPF (10−5 M) in the presence of concanavalin A (Con A) (5 µg/mL, T lymphocytes), control was also stimulated with Con A; (B) MT (10−5 M) or CPF (10−5 M) in the presence of lipopolysaccharide (LPS) (10 µg/mL, B lymphocytes), control was also stimulated with LPS; (C) MT (10−5 M) alone or different concentrations (1, 5, and 10 µg/mL) of WPE together with MT in the presence of Con A (T lymphocytes); (D) CPF (10−5 M) alone or different concentrations (1, 5, and 10 µg/mL) of WPE together with CPF in the presence of LPS (B lymphocytes). Results are presented as mean ± SD of three separate experiments. * p < 0.05 or ** p < 0.01 vs. untreated control; # p < 0.05 or ## p < 0.01 vs. MT or CPF treatment.