Fig. 4.

Comparison of elution programs applied to Dox1. AF4 fractograms resulting from different elution programs (normalized excess Rayleigh ratio at 90°), R_g (line) and the R_h (dots) as a function of elution time for injection of 25 μg of Dox1. Constant XF = 0.3 (black), exponential decay from XF = 1.0 to 0 in 60 min (red) and linear decay from XF = 1.0 to 0 in 30 min (blue). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)