Table 1.
Optimized AF4 fractionation method for liposomal formulations.
| Channel parameters | Channel length |
Long channel (280 mm) |
|
|---|---|---|---|
| Spacer | 350 μm with wide width | ||
| Membrane | Type | Regenerated cellulose (RC) | |
| MWCO | 10 kDa | ||
| Flow rates | Injection flow | 0.2 mL/min | |
| Channel flow | 0.5 mL/min | ||
| Focus flow | 2 mL/min | ||
| Cross flow | 0.3 mL/min | ||
| Sample loading | Injection amount | 25 μg | |
| Time and flow parameters (as sequenced in the method) | Mode | Step duration (min) | XF (mL/min) |
| (1) Elution | 2 | 0 | |
| (2) Focus | 2 | – | |
| (3) Focus + Injection | 8 | – | |
| (4) Elution | 45 | 0.3 | |
| (5) Elution | 15 | 0 | |