The intestinal barrier is maintained by AC in ob/ob mice. (A) Relative mRNA expression of tight junction proteins and CD36 between ob+/+-Ctrl and ob/ob-Ctrl mice; (B) The intestine was examined using hematoxylin and eosin staining and immunohistochemical staining of CD36. Magnification, 40×. Scale bars are 20 μm. The negative control for immunohistochemistry has been marked NC; (C) The intestine was examined using immunohistochemical staining for ZO-1. Magnification, 40× and 100×; scale bars are 50 μm and 10 μm, respectively. All data are expressed as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001. Non-significant; ns. n = 4–8 mice in each group.