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. 2020 Feb 28;12(3):641. doi: 10.3390/nu12030641

Figure 4.

Figure 4

Liver Sqstm1 mRNA (A). Liver protein levels (arbitrary units) of p-mTOR/mTOR (B), p-p70S6K/p70S6K (C), p-ULK1/ULK1 (D), Beclin 1 (E) and LC3BII/LC3BI (F). Data correspond to the mean ± SE of n = 22 rats. When necessary, blots were stripped using Restore™ Plus Western Blot Stripping Buffer (Thermo Scientific, Waltham, MA, USA) and reprobed with antibodies that recognize the total form of a protein or control proteins. CT: sedentary rats without gavage after 10 h of fast (n = 4). EXE-imm: after 10 h of fast, rats were submitted to the resistance exercise protocol and received water gavage immediately after exercise (n = 4). EXE-1h: after 10 h of fast, rats were submitted to the resistance exercise protocol and received water gavage 1 h after exercise (n = 4). EXE/Suppl-imm: after 10 h of fast, rats were submitted to the resistance exercise protocol and received a protein blend gavage immediately after exercise (n = 5). EXE/Suppl-1h: after 10-h of fast, rats were submitted to the resistance exercise protocol and received a protein blend gavage 1 h after exercise (n = 5). a p ≤ 0.05 versus EXE/Suppl-1h; e p ≤ 0.05 versus CT, f p ≤ 0.05 versus EXE-imm.