Figure 3.

Effect of domain deletion on TRIM62 expression. The relative mRNA expression of TRIM62 in CEF cells transfected with RING (A), B-box (B), coiled-coil (C), or SPRY (D) domain deletion mutant with or without REV infection were measured by qRT-PCR using pEmpty and pTRIM62 as controls. (E) TRIM62 protein expression levels were detected with specific primary antibodies against Flag label and TRIM62 by Western blotting, respectively. pEmpty represent empty vector, pTRIM62 represent TRIM62 full length, pTRIM62-ΔR represent RING domain deletion, pTRIM62-ΔB represent B-box domain deletion, pTRIM62-ΔC represent coiled-coil domain deletion, and pTRIM62-ΔS represent SPRY domain deletion. *p < 0.05, **p < 0.01, and ***p < 0.001.