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. 2020 Apr 3;7:152. doi: 10.3389/fvets.2020.00152

Figure 4.

Figure 4

Inhibition of REV RNA expression in CEF cells by TRIM62 RING, B-box, coiled-coil, and SPRY domain deletion mutants. The relative mRNA expression levels of REV in CEF cells transfected with RING, B-box, coiled-coil, or SPRY domain deletion mutants were measured by qRT-PCR and Western blotting. pEmpty (A) and pTRIM62 (B) were used as controls. pEmpty represent empty vector, pTRIM62 represent TRIM62 full-length, pTRIM62-ΔR represent RING domain deletion, pTRIM62-ΔB represent B-box domain deletion, pTRIM62-ΔC represent coiled-coil domain deletion, and pTRIM62-ΔS represent SPRY domain deletion. *p < 0.05, **p < 0.01, and ***p < 0.001.