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. 2020 Feb 4;15(2):325–333. doi: 10.1021/acschembio.9b00963

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Copyright © 2020 American Chemical Society

This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.

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Successful engagement of recombinant but not endogenous PARP6 in lysate-based crosslinking experiments. In-gel fluorescence (top) and immunoblot (bottom) analysis of lysates labeled with PARPYnD and ligated to AzTB with/without cotreatment with AZ0108. Left panel—lysates were spiked with recombinant GST-PARP6 before treatment. Ligated samples were further enriched on streptavidin beads and all samples analyzed as above: > GST-PARP6 (98 kDa); * PARP6 (71 kDa). More recombinant protein is labeled/enriched compared to vehicle (DMSO) control, where residual signal can be observed through nonspecific interaction of the recombinant protein with the beads. No enrichment of endogenous PARP6 is observed.