Figure 3.

(A) Structures of ZnPD5 and its Zn(II)-catalyzed release of GNF-4877. (B) Reaction kinetics of ZnPD5 with different concentrations of Zn(II) as monitored by fluorescence spectroscopy. (C) Fold change in fluorescence in INS1E cells versus other cells. (D) Representative images of ZnPD5-treated cells under the FITC channel (top), DAPI staining (middle), and the overlay (bottom). (E) Representative fluorescence images of intact human islets showing β-cell selective hydrolysis of ZnPD5 under the FITC channel (green) and C-peptide (red). Intact islet cells were treated with either DMSO (top) or 150 nM of ZnPD5 (bottom). (F) Dose-dependent induction of β-cell proliferation by ZnPD5 in human islets.