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. 2020 Apr 10;18:61. doi: 10.1186/s12964-020-00550-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — miR-10b is upregulated in RDEB-cSCC. a Unsupervised clustering via PCA on miRNA expression levels clearly separated RDEB-cSCC (n = 4) and HC-cSCC (n = 3) samples from RDEB-KC (n = 6) and HC-KC (n = 5). Target analysis of miR-10b, which was significantly (p ≤ 0.05) up-regulated (≥ 2-fold) in both, b HC-cSCCs and (c) RDEB-cSCCs, indicated an enrichment of pathways related to aggressive tumor phenotypes like epithelial-to-mesenchymal transition (EMT) (d). e TaqMan qPCR (n = 3 ind. repl., mean ± SEM, *p < 0.05, n.s. non-significant, unpaired t-test), as well as (f) fluorescence-based in situ hybridization (FISH) with DIG labelled miR-10b probes (green) on FFPE tissues sections of confirmed (H&E staining, top row) carcinomas also showed miR-10b dysregulation. White dashed line: tumor boundaries marked by pan-keratin staining (red). Scale bars: 100 μm (white), 500 μm (black). Sample codes (e) from left to right HC-KC: 1090KC, SKC013, SKC018; RDEB-KC: RDEB-55KC, RDEB-43KC, RDEB-29KC; RDEB-cSCC: RDEB-SCC1, RDEB-SCC2, RDEB-SCC62; HC-cSCC: SCC13, WT18SCC, A431