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. 2020 Apr 9;20:116. doi: 10.1186/s12935-020-01167-1

Table 1.

Primer sequences for RT-qPCR

Gene Forward sequence Reverse sequence
LncRNA GAS5 5′-TCTGAGCAGGAATGGCAGTGT-3′ 5′-CATCCTCCTTTGCCACAGAAC-3′
EZH2 5′-TCGAGCTGCTCTGCTCTCTA-3′ 5′-CTTGAGCTGTCTCAGTCGCA-3′
CDKN1C 5′-CGTTCTTCTCGGGTGGA-3′ 5′-CTGTACTCACTTGGCTCA-3′
GAPDH 5′-TGTTCGTCATGGGTGTGAAC-3′ 5′-ATGGCATGGACTGTGGTCAT

RT-qPCR, reverse transcription quantitative polymerase chain reaction; LncRNA GAS5, long non-coding RNA growth arrest-specific transcript 5; EZH2, enhancer of zeste homolog 2; CDKN1C, cyclin-dependent kinase inhibitor 1C; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; R, reverse