Figure 1.

Application of design of experiments (DoE) to modulate biosensor dose response curves. (A) An experiment can be considered as a point in multidimensional space. DoE is a statistical tool that enables the proper exploration of experimental space to understand and optimize biology. (B) Modulation of biosensor dose response curves by increasing maximum output in the ON state while minimizing output in the OFF state (vertical extension; left), increasing sensitivity (middle), and conversion of a digital response to an analogue one (horizontal extension; right).