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. 2020 Apr 9;26:32. doi: 10.1186/s10020-020-00153-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Genes regulated by D2 and D3 and relative effect of differentiation alone. The numbers of significant probes are indicated in each pie chart. The top pie charts in all panels indicate the number of probes that are up- or down-regulated by vitamin D2 (a and c) or D3 (b and d) compared to the control at the same time point (a and b, 24 h; c and d, 72 h). The bottom pie charts of all panels describe how these genes are regulated by differentiation (defined by comparing Ctrl 72 h vs Ctrl 24 h). Differentially regulated genes were obtained according to the same criteria described in the legend to Fig. 2a. All the probes-genes are included in Additional File 1, where the membership of the pie chart groups is reported