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. 2020 Apr 9;39:61. doi: 10.1186/s13046-020-01551-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Linc01134 functions as ceRNA to up-regulate IGF2BP1 via sponging miR-324-5p. a MiRNAs that can bind to linc01134 were predicted by starBase and DIANA public databases. b qRT-PCR was detected the level of miRNAs after silencing linc01134. c The binding site between miR-324-5p and linc01134 was predicted by starBase. d Subcellular fractionation and FISH assays were conducted to understand the subcellular location of linc01134. e Luciferase reporter measured the luciferase activity of linc01134-WT/MUT under miR-324-5p mimics. f RNA pull down verified physical interaction between linc01134 and miR-324-5p. g qRT-PCR measured the expression of the indicated mRNAs after overexpressing miR-324-5p. h qRT-PCR assay was performed to examine the expression of IGF2BP1 in HCC tissues and para-tumor tissues. i Pearson correlation was performed to explore the correlation between linc01134 and IGF2BP1. j Potential binding site between miR-324-5p and IGF2BP1 was predicted by starBase. k-l Luciferase reporter and RIP verified the binding relation between IGF2BP1 and miR-324-5p. m The transfection efficiency of pcDNA3.1/linc01134 was detected by qRT-PCR. n RIP-based PCR evaluated the relative enrichment of linc01134 and IGF2BP1 in anti-Ago2 group. o qRT-PCR and western blot were conducted to measure the mRNA and protein levels of IGF2BP1 in transfected cells. *P < 0.05, **P < 0.01