Fig. 2.

Macrophage polarization was modulated by Ern1. a Ern1 expression in macrophages transfected with different plasmids detected by RT-qPCR. b Ern1 expression in macrophages transfected with different plasmids determined by western blot analysis. c Arg1, Ym1, and Fizz expression in differently treated macrophages measured by RT-qPCR. d Arg1, Ym1, and Fizz expression in differently treated macrophages examined by western blot analysis. e M2 macrophage proportion in differently treated macrophages identified by flow cytometry. *p < 0.05 versus control. The measurement data were presented as mean ± standard deviation. One-way ANOVA was applied to compare differences between multiple groups, followed by a Tukey’s post hoc test. The cell experiment was repeated thrice