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. 2020 Apr 9;11:152. doi: 10.1186/s13287-020-01640-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Adipogenesis assay (a, b) and osteogenesis assay (c, d) results. Gene expression of FABP4 relative to undifferentiated monolayer cells on day 21 (a), visualization of oily droplets in representative TCOA and TCA cultures by Oil Red O staining (b), gene expression of Runx2 relative to undifferentiated monolayer cells on day 21 (c), and visualization of calcium nodules and calcified matrices in representative TCOA and TCO cultures by Alizarin Red S staining (d). Lines and asterisk indicate groups with significant differences. Images are at a × 10 magnification. T, tenogenesis positive; C, chondrogenesis positive; O, osteogenesis positive; A, adipogenesis positive