Table 3.
Segregation of BC2F2 progeny of heterozygous alien translocation plants into rust resistant and homozygous rust resistant plants
| Line | No. of BC2 plants | No. of BC2F2 progeny plants | Heterozygous Resistant | Homozygous Resistant | Χ2 * | ||||
|---|---|---|---|---|---|---|---|---|---|
| No. of plants | % of plants | No. of plants | % of plants | ||||||
| Observed | Expected | Expected | Observed | ||||||
| R-1 | 3 | 257 | 152 | 128.5 | 59.1 | 39 | 64.25 | 15.2 | 14.27 |
| R-2 | 2 | 190 | 113 | 95 | 59.5 | 17 | 47.5 | 8.9 | 26.28 |
| R-3 | 2 | 215 | 128 | 107.5 | 59.5 | 28 | 53.75 | 13.0 | 16.76 |
| R-4 | 2 | 188 | 107 | 94 | 56.9 | 10 | 47 | 5.3 | 43.18 |
| R-5 | 3 | 274 | 173 | 137 | 63.1 | 0 | 68.5 | 0 | 93.38 |
| R-6 | 4 | 396 | 255 | 198 | 64.4 | 2 | 99 | 0.5 | 127.61 |
| R-7 | 1 | 95 | 47 | 47.5 | 49.4 | 12 | 23.75 | 12.6 | 12.14 |
| R-8 | 3 | 285 | 187 | 142.5 | 65.6 | 45 | 71.25 | 15.7 | 28.24 |
| R-9 | 2 | 197 | 111 | 98.5 | 56.3 | 24 | 49.25 | 12.2 | 17.83 |
| R-10 | 2 | 190 | 115 | 95 | 60.5 | 34 | 47.5 | 17.8 | 8.94 |
| R-20 | 2 | 188 | 134 | 94 | 71.3 | 21 | 47 | 11.1 | 35.57 |
| R-30 | 2 | 155 | 79 | 77.5 | 51.0 | 1 | 38.75 | 0.6 | 70.72 |
Resistance to leaf rust pathogen was determined at seedling stage. PCR marker Zyg_1Sh_1 was used to determine presence of Ae. sharonensis resistance segment, markers Zyg_1Sh_1 and Zyg_2G_2 determined zygosity of the plants. Expected values are 50% heterozygous, 25% homozygous resistant and 25% homozygous susceptible plants
*All Χ2 values present significant (p > 0.05) deviation from the expected distribution