Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 2;219(4):e201908225. doi: 10.1083/jcb.201908225

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Levic et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — A low temperature block synchronizes biosynthetic transport of p75 in the TGN in vivo. (A–E) A low temperature TGN block for zebrafish larvae. (A) Schematic of low-temperature TGN block. (B and C) Apical membrane transport (arrow) of newly synthesized p75-GFP is stalled after a 2-h low temperature block. Arrowheads point to perinuclear pool. n ≥ 5 larvae per condition in three independent experiments. Scale bars are 10 µm. (D) Newly synthesized p75-GFP is retained at the TGN (arrowheads), marked by Galt-tagRFPt. n = 5 larvae. (E) Colocalized p75-GFP⁺ and B4GALT1-tagRFPt⁺ pixels from D. Scale bars are 10 µm. (F–H) A TGN block and release assay for zebrafish larvae. (F) Experimental strategy. (G) p75-GFP localization at various time points after a TGN release. p75-GFP is rapidly released from the TGN into apical carriers, progressively accumulating at the apical membrane. Scale bars are 10 µm. (H) Kinetic analysis of p75-GFP transport after a TGN release plotted as a ratio of apical membrane intensity (C_AM) to cytoplasm intensity (C_C) over time. Data are fitted with a one-phase exponential association model (R² = 0.8159; n > 20 cells from at least two animals per time point; error bars are SD).