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Live imaging of mRFP-Rab8a and p75-GFP after a 45-min TGN release. 5-dpf larvae were subjected to a 3-h TGN block, mounted in low melting point agarose in glass-bottom dishes, and then transferred to a 28°C incubation chamber. Live imaging of the mid-intestine was started ~45 min after TGN release. Images were acquired at 1 frame/4 s. Time stamps are shown in the video. White rectangles indicate the TGN region of enterocytes.
