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Effects of V-ATPase inhibition on mRFP-Rab8a dynamics. 5-dpf larvae were mounted in low melting point agarose in glass-bottom dishes and then covered in fresh media containing either 1% DMSO (control) or 1 µM bafilomycin. Live imaging of the mid-intestine was started ~45–50 min after adding media. Images were acquired at 1 frame/4 s. Time stamps are shown in the video. Tracks of mRFP-Rab8 particles were assigned using mTrackJ in ImageJ.
