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. 2020 Feb 24;219(4):e201907149. doi: 10.1083/jcb.201907149

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© 2020 Nijenhuis et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 7. — Application of the optimized optogenetic toolbox to endogenously tagged cargo. (A and B) Fixed-cell imaging (A) and quantification (B) of recycling endosomes, endogenously tagged with iLID-mCherry-RAB11 and transiently expressed SSPB(nano)-GFP-GCN4–ppKin14-VIb in HeLa cells, in the dark or after 30 min of blue light. Insets show 2.5-fold magnifications of the MTOC region. (B) Quantification shows the normalized enrichment of RAB11 at the MTOC relative to nonilluminated cells. Dots represent one cell and bars indicate mean and 95% confidence intervals. Data were from three independent experiments. Scale bars are 10 µm. Asterisks indicate significance (Student’s t test, unpaired). ∗∗∗∗, P < 0.0001.