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. 2020 Feb 27;219(4):e201907018. doi: 10.1083/jcb.201907018

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© 2020 Deng et al.

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Figure 6. — Myr-WASp promotes the recruitment of α-Spec to the cell cortex. All images are z-projections at lateral position. (A and A’) A Triton X-100–permeabilized pupal eye disc containing GFP-positive MARCM clones with Myr-WASp overexpression was stained for phalloidin. Note the increased cortical F-actin level in the overexpression clones. Quantification of phalloidin membrane intensity is shown in F. (B and B’) Similar to A and A’ except that α-Spec staining was examined. Note the increased cortical α-Spec level in the overexpression clones. Quantification of α-Spec membrane intensity is shown in G. (C and C’) A Triton X-100–permeabilized pupal eye disc containing GFP-positive hts^null mutant MARCM clones with Myr-WASp overexpression was stained for phalloidin. Note the decreased cortical F-actin level in hts^null mutant clones with Myr-WASp overexpression. Quantification of phalloidin membrane intensity is shown in F. (D and D’) Similar to C and C’ except that α-Spec staining was examined. Note the decreased cortical α-Spec level in hts^null mutant clones with Myr-WASp overexpression. Quantification of α-Spec membrane intensity is shown in G. (E and E’) A pupal eye disc containing GFP-positive arpc1^Q25sd mutant MARCM clones was stained for α-Spec. Note the decreased cortical α-Spec level in arpc1 mutant clones. (F and G) Normalized mean membrane intensity of α-Spec or phalloidin staining for the indicated genotypes. Data are means ± SEM (n ≥ 20 cells, representative of five animals). ****, P < 0.0001. Scale bars, 5 µm.