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. 2020 Feb 27;219(4):e201907018. doi: 10.1083/jcb.201907018

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© 2020 Deng et al.

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Figure S1. — Further analysis of the coupling between cell shape and myosin II activity in wild-type and spectrin mutant PECs. All images are z-projections at AJs. Pupal eye discs containing GFP-positive MARCM clones of the indicated genotypes were stained for p-MLC (red) and DE-cad (green) in A–A”’ or DE-cad (red) in B–I’. Arrowheads indicate mutant 1° PEC and arrows indicate wild-type 1° PEC from the same mosaic ommatidium. PECs with Rok^CAT overexpression show increased p-MLC and decreased apical size compared with wild-type PECs (compare arrow and arrowhead in A”). Also note the extra interommatidial cells (yellow asterisks in A”) in Rok^CAT overexpression clones. 20 ommatidia were used for counting interommatidial cells, and the number on the lower left in A” indicates the number of extra cells per cluster. See Fig. 1 H for quantification of apical area of 1° PEC analyzed in A–I. Scale bars, 5 µm.