Figure 1.

Loss of Setx does not affect oocyte maturation in young mice. (a) Quantification of fully grown GV-stage oocytes per mouse (six mice per genotype). (b and c) Representative images of γH2AX and DNA labeling in fully grown Setx^+/+ and Setx^−/− oocytes (b) and quantification of γH2AX-intensity per oocyte (c). (d and e) GVBD (d) and first polar body extrusion (PBE; e) rates of oocytes from Setx^+/+ and Setx^−/− mice (four mice per genotype). (f) Representative time-lapse series of young Setx^+/+ and Setx^−/− oocytes at GV, shortly after GVBD, followed by metaphase I, anaphase I, and metaphase II arrest (M-II). Black arrows depict the first polar body. Scale bars, 10 µm. Oocyte numbers are shown in parentheses from a minimum of three independent experiments. Error bars are mean ± SEM. Two-tailed Student’s t test (a and c) or two-way ANOVA (d and e) was used for statistical analysis; ns, P > 0.05.