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. 2020 Mar 30;219(4):e201907213. doi: 10.1083/jcb.201907213

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© 2020 Subramanian et al.

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Figure 5. — The DDR in oocytes involves increased proteolysis. (a–c) Quantification of cyclin B1-GFP fluorescence decline in Setx^+/+-ON and Setx^−/−-ON (a), Setx^+/+-immediate and Setx^−/−-immediate (b), and Eto-immediate and Eto-ON (c) GV-stage oocytes. (d and e) Representative immunoblot (d) and quantification of cyclin B1 band intensities (e) in Setx^+/+-ON and Setx^−/−-ON oocytes (30 oocytes per lane). (f and g) Representative immunoblot (f) and quantification of cyclin B1 band intensities (g) in Eto-immediate and Eto-ON oocytes (30 oocytes per lane). (h and i) GVBD rates for Setx^+/+-ON (h) and Eto-ON (i) oocytes microinjected with either RFP or ND-Cyclin B1-RFP cRNA. Oocyte numbers are shown in parentheses from a minimum of three independent experiments. Error bars are mean ± SEM. Two-tailed Student’s t test (e and g) or two-way ANOVA (a, b, c, h, and i) was used for statistical analysis. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001; ns, P > 0.05. In immunoblots, vinculin served as a loading control.

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