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. 2020 Mar 13;219(4):e201911129. doi: 10.1083/jcb.201911129

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© 2020 Smith et al.

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Figure S1. — Sequences of fxr1 exon 15 from sgRNA-treated embryos. Sequences of fxr1.S and fxr1.L from five randomly selected X. laevis embryos injected with the sgRNA T1 (green) and T3 (underlined). sgRNA-injected embryos had deletions (dashed), uncallable nucleotides (N), and mismatches (red) compared with reference sequences. Deletions roughly correspond to 3–4 nt upstream of the protospacer adjacent motif (PAM) sequences (magenta). Fig. S1 is linked to Fig. 1, E and F.