Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 12;219(4):e201910084. doi: 10.1083/jcb.201910084

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Dattoli et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — CID deposition in GSCs requires CYCA, CYCB, and HASPIN. (A–H) Wild-type germaria stained for DAPI (cyan), anti-CID (red), and anti-CYCA or anti-CYCB (green). (I–N″) Confocal z-stack projection of nanos-Gal4 (I–J″), CYCA RNAi (K–L″), CYCB RNAi (M–N″) germaria, stained for DAPI (cyan), anti-VASA (yellow), anti-CID (green), and anti-1B1 (spectrosome, red). (O) Quantification of CID fluorescence intensity at centromeres per nucleus (L). (P–S″) Confocal z-stack projection of nanos-Gal4 (P–Q″) and HASPIN RNAi germaria (R–S″), stained for DAPI (cyan), anti-VASA (yellow), anti-CID (green), and anti-1B1 (spectrosome, red). (T) Quantification of CID fluorescence intensity (MGVs) at centromeres per nucleus. Data are represented as the mean ± SEM; ***, P < 0.0005; *, P < 0.05, calculated with unpaired t test with Welch’s correction. Star indicates the terminal filament and arrows indicate centromeres; 3-d-old female flies; scale bar, 10 µm; inset, 5 µm.