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. 2020 Mar 12;219(4):e201910084. doi: 10.1083/jcb.201910084

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© 2020 Dattoli et al.

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Figure S3. — Chromosomes retain differential amounts of CID and CENP-C upon centromere assembly in Drosophila female GSCs. (A–N′) Superresolution SIM z-stack projection of a Drosophila GSC of a wild-type germarium in prometaphase and metaphase, stained for DAPI (blue), anti-CID (red), anti-H3T3P (green), and anti-SPECTROSOME (magenta). (O) Diagram of Drosophila germarium, highlighting the four-cell cyst stage containing four CCs. (P) Superresolution SIM z-stack projection of a germarium capturing four CCs in prometaphase/metaphase, which divide synchronously. (Q–R′) Superresolution SIM z-stack projection of a CC at prometaphase. (T) Table of the ratio values obtained for each cell analyzed. (U–Y′) Confocal z-stack projection of a GSC of a wild-type germarium in prometaphase and metaphase, stained for DAPI (cyan), anti-TUBULIN (red), and anti-H3T3P (green). White line highlights the cap cells; arrowheads, spectrosome; arrows, fusome (not yet visible in the z-stacks projected); scale bar, 5 µm; inset, 1 µm.