Figure S3.

DNA loops and TADs observed in mouse oocytes are largely dependent on Scc1-cohesin. (A) Representative images of in situ fixed Scc1^fl/fl, Scc1^Δ/Δ, Wapl^fl/fl, and Wapl^Δ/Δ GV-oocytes in NSN state (immature, NSN). A single Z plane is shown to better visualize vermicelli structures in Wapl^Δ/Δ oocytes. DNA is shown in blue, and Smc3 and Scc1 in gray. Scale is the same in all images; scale bar, is 10 µm. (B) Average loops, TADs, and compartmentalization in Scc1^fl/fl, Scc1^Δ/Δ, Wapl^fl/fl, and Wapl^Δ/Δ GV-oocytes in NSN state. The number of oocytes analyzed is indicated in the figure. Three Wapl^fl/fl, four Wapl^fl/fl (Tg)Zp3-Cre, two Scc1^fl/fl, and two Scc1^fl/fl (Tg)Zp3-Cre littermate females were analyzed. (C) P_c(s) for Scc1^fl/fl, Scc1^Δ/Δ, Wapl^fl/fl, and Wapl^Δ/Δ GV-oocytes in NSN state. Slops of the log(P_c(s)) curves for each condition are shown below the P_c(s) plots. Gray lines show the controls Scc1^fl/fl (left panel) and Wapl^fl/fl (right panel), and blue lines show Scc1^Δ/Δ (left panel) and Wapl^Δ/Δ (right panel). (D) Quantification of the number of contacts within loop coordinates. The average number of contacts observed per loop is represented. Statistical significance was tested using a paired Wilcoxon rank-sum test (n.s., not significant). The number of oocytes analyzed is the same as in B.